Statistical Evaluation of HTS Assays for Enzymatic Hydrolysis of β-Keto Esters
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منابع مشابه
Statistical Evaluation of HTS Assays for Enzymatic Hydrolysis of β-Keto Esters
β-keto esters are used as precursors for the synthesis of β-amino acids, which are building blocks for some classes of pharmaceuticals. Here we describe the comparison of screening procedures for hydrolases to be used for the hydrolysis of β-keto esters, the first step in the preparation of β-amino acids. Two of the tested high throughput screening (HTS) assays depend on coupled enzymatic react...
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ژورنال
عنوان ژورنال: PLOS ONE
سال: 2016
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0146104